Home | About | Quick Start | FAQ
Beta Version 0.1
DHARA is an online index of articles on Ayurveda published in research journals worldwide. Users Online: 665
  Enter Keyword Below :
      
Advanced

Journal of Ethnopharmacology 2014 May ; (3) :0
Cytochrome P450 inhibitory potential and RP-HPLC standardization of trikatu--a Rasayana from Indian Ayurveda.

Abstract
Trikatu is a very well known 'Rasayana' in Ayurveda and widely used as a polyherbal ayurvedic formulation in India. It consists of three well known plants, viz., Piper longum (PL), Piper nigrum (PN) and Zingiber officinale (ZO) in equal ratio. Trikatu has been prescribed for cough, cold, fever, asthma, respiratory problems and improvement of digestive disorders. The aim of the present study was to investigate the effect of individual ingredients of trikatu namely PL, PN, and ZO and formulations [Marketed formulation (MF) and laboratory formulation (LF)] on drug metabolizing enzymes (CYP3A4 and CYP2D6), to assess its herb-drug interaction potential through cytochrome P450 inhibition assays. Further this work was aimed to develop an RP-HPLC method for the identification and quantification of piperine and 6-gingerol in the crude drug trikatu.Enzyme inhibition effect of LF, MF, PL, PN and ZO was explored through CYP450-CO complex assay using rat liver microsomes (RLM) and a fluorescence screening method using individual isoenzymes (CYP3A4 and CYP2D6). The RP-HPLC method was developed for the identification and quantification of piperine and 6-gingerol in LF, MF and individual plant materials at the concentration of 1mg/mL.RP-HPLC analysis confirmed the presence of piperine and 6-gingerol in LF and MF [Piperine: 7.89±2.12% (w/w) (MF), 6.70±2.13% (w/w) (LF)]; [6-gingerol: 5.3±1.21% (w/w) (MF), 4.95±2.34% (w/w) (LF)]. Inhibitory potential of MF and LF in CYP450-CO complex assay was found to be 37.54±3.12% (MF) and 35.12±2.31% (LF) and against CYP2D6 and CYP3A4 was estimated to be IC50 251.30±3.98 and 245.23±1.92µg/mL and IC50 225.50±1.02 and 223.254±0.92µg/mL respectively.Different concentrations of the trikatu formulation and its individual components showed significantly (p<0.001) less inhibitory activity on individual isoenzymes as compared to the positive control. The crude drug exhibited inhibitory potential against the CYP450 enzymes in a concentration dependent manner. Outcome of the present study demonstrated that trikatu has less interaction potential with drug metabolizing enzymes.Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

DHARA ID: D054654 Pubmed ID: 24690772


Access to Full Paper Not Available

Replica Louis vuitton Belts Replica Louis vuitton Handbags Replica Louis vuitton Backpack Replica Louis vuitton Capucines Bags Imitazioni Louis vuitton Borse Imitazioni Louis vuitton Cinture Imitazioni Louis vuitton Occhiali Replica Louis vuitton Geldb?rsen Replica Louis vuitton Gürtel Replica Louis vuitton Schmuck Replica Louis vuitton Schal Imitation Louis vuitton Bijoux Imitation Louis vuitton Ceinture Imitation Louis vuitton Chaussures Imitation Louis vuitton Foulard namaak Louis vuitton Gordels namaak Louis vuitton Handtassen namaak Louis vuitton Portemonnees namaak Louis vuitton Schoenen Replica Louis vuitton Bolsos Replica Louis vuitton Billeteras Replica Louis vuitton Cinturones Dior Replica Hermès Replica Yves Saint Laurent Replica Goyard Replica Imitation Louis Vuitton Imitation Hermès Imitation Dior Imitation Yves Saint Laurent Imitazioni Bottega Veneta Borse Imitazioni Delvaux Borse Imitazioni Goyard Borse Replica Taschen Replica Louis Vuitton Replica Hermès Replica Dior Replica Bottega Veneta tassen Replica Chloé tassen Replica Delvaux tassen Replica Goyard tassen imitacion Louis Vuitton imitacion Louis Vuitton imitacion Hermès imitacion Dior Replica Miu Miu Replica Prada Replica Yves Saint Laurent Replica Chanel Replica Gucci Replica Hermès Replica Miu Miu Réplique Chanel Réplique Hermès Réplique Miu Miu Imitazioni Chanel Imitazioni Hermès Imitazioni Miu Miu imitacion Bolso Yves Saint Laurent imitacion Hermes imitacion Chanel imitacion Gucci Gucci Replica Hermes Replica

Copyright | Disclaimer | Feedback | Updates | Contact
Developed and maintained by AVP Research Foundation (Formerly AVT Institute for Advanced Research), 136/137, Trichy Road, Ramanathapuram, Coimbatore - 641045, Tamil Nadu, India
Funded by Central Council for Research in Ayurvedic Sciences, Dept. of AYUSH, Ministry of Health and Family Welfare, Government of India, New Delhi