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Environmental science and pollution research international
2011
Sep
;
(8)
:0
A possible new mechanism involved in ferro-cyanide metabolism by plants.
Yu XZ
,
Li F
,
Li K
Abstract
Ferro-cyanide is one of the commonly found species at cyanide-contaminated soils and groundwater. Unlike botanical metabolism of KCN via the ß-cyanoalanine pathway, processes involved in the plant-mediated assimilation of ferro-cyanide are still unclear. The objective of this study was to investigate a possible mechanism involved in uptake and assimilation of ferro-cyanide by plants.Detached roots of plants were exposed to ferro-cyanide in a closed-dark hydroponic system amended with HgCl(2), AgNO(3), LaCl(3), tetraethylammonium chloride (TEACl), or Na(3)VO(4), respectively, at 25?±?0.5°C for 24 h. Total CN, free CN(-), and dissolved Fe(2+) were analyzed spectrophotometrically. Activity of ß-cyanoalanine synthase involved in cyanide assimilation was also assayed using detached roots of plants in vivo.Dissociation of ferro-cyanide [Fe(II)(CN)(6)](-4) to free CN(-) and Fe(2+) in solution was negligible. The applied inhibitors did not show any significant impact on the uptake of ferro-cyanide by soybean (Glycine max L. cv. JD 1) and hybrid willows (Salix matsudana Koidz × alba L.; p?>?0.05), but rice (Oryza sativa L. cv. JY 98) was more susceptible to the inhibitors compared with the controls (p?0.05). However, TEACl had the most severe effect on the assimilation of ferro-cyanide by soybean, hybrid willows, and maize (Zea mays L. cv. PA 78; p?0.01), whereas AgNO(3) was the most sensitive inhibitor to rice (p?0.01). No measurable difference in ß-cyanoalanine synthase activity of roots exposed to ferro-cyanide was observed compared with the control without any cyanides (p?>?0.05), whereas roots exposed to KCN showed a considerable increase in enzyme activity (p?0.05).Plants take up Fe(2+) and CN(-) as a whole complex, and in vivo dissociation to free CN(-) is not prerequisite during the botanical assimilation of ferro-cyanide. Ferro-cyanide is likely metabolized by plants directly through an unknown pathway rather than the ß-cyanoalanine pathway.
DHARA ID:
D015719
Pubmed ID:
21465162
Access to Full Paper Not Available
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